Form:Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, <0.02% sodium azide, 50% glycerol.
Specificity:Recognizes endogenous levels of the large fragment (17/19kD) of activated human Caspase 3 resulting from cleavage adjacent to Asp175. Does not recognize full length Caspase 3 or other cleaved caspases. Non-specific labeling may be observed by immunofluorescence in specific sub-types of healthy cells in fixed-frozen tissues (e.g. pancreatic alpha-cells). Cytplasmic background may be observed in human and monkey samples. Species Crossreactivity: mouse, rat and monkey
Clone # USB:9j115(5A1E)
Isotype:IgG
Calc Applications Abbrev:FC IC IF IHC IP WB
Calc Crossreactivity:Hu Mk Mo Rt
Immunogen:Synthetic peptide corresponding to N-terminal residues adjacent to Asp175 in human Caspase 3. Species Sequence Homology: canine