Purity:Purified by Protein A affinity chromatography.
Form:Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Specificity:Specific for cyclin E. The epitope is located within the last 80 aa of the C-terminal (1) . HE12 antibody detects a ~50kD band on human HeLa cell lysates by Western blot analysis. Reacts with human cyclin E (HeLa cells). Reactivity with other species has not been tested. USAGE The dilutions listed below are good starting points. However, optimal dilutions should be determined by the investigator for each application. Western Blotting (1) : 1 – 2 µg/ml Immunoprecipitation (1) : 1 µg/IP Flow cytometry (8,9) : Immunohistochemistry (10) : Gel Mobility Shift Assay:
Clone # USB:HE12
Isotype:IgG1,k
Calc Applications Abbrev:FC IHC IP WB
Calc Crossreactivity:Hu
Immunogen:Full-length, recombinant, human cyclin E protein.