Purity:Purified by Protein A affinity chromatography.
Form:Supplied as liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol and less than 0.02% sodium azide.
Specificity:Recognizes mouse phospho-threonine only when followed by proline. It reacts with proteins/peptides phosphorylated on the Thr-Pro motif in an otherwise highly context-independent fashion. The phosphospecific antibody does not recognize phospho-threonine in the absence of an adjacent proline. Does not react with phospho-tyrosine, but does react with some phospho-serine peptides containing the phospho-serine-proline motif (e.g., phospho-Elk-1). Species Crossreactivity: all species.
Clone # USB:3A40(P-Thr-Pro-101)
Isotype:IgM
Calc Applications Abbrev:E IHC WB
Calc Crossreactivity:Mo
Immunogen:Synthetic phosphothreonine-proline-containing peptides. Antibody is a mouse IgM clone and can be recognized by anti-mouse Ig (whole molecule) secondary antibody.