Form:Supplied as a liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 0.02% sodium azide, 50% glycerol.
Specificity:Recognizes endogenous levels of human p44 and p42 MAP kinase (Erk1 and Erk2) when dually phosphorylated at Thr-202 and Tyr-204 of Erk1 (Thr-185 and Tyr-187 of Erk2) and singly phosphorylated at Try-204. Does not crossreact with the corresponding phosphorylated residues of either SAPK/JNK or p38 MAP kinase. This antibody may cross-react with an unknown cytoskeletal protein in some cell lines as visualized by immunofluorescence.Species Crossreactivity: Hamster, mouse, rat and zebrafish.
Clone # USB:3A22(E10)
Isotype:IgG1
Calc Applications Abbrev:E FC IC IP WB
Calc Crossreactivity:Hm Hu Mo Rt
Immunogen:Synthetic phosphopeptide corresponding to residues around Thr202/Tyr204 of human p44 MAP kinase.