Specificity:Recognizes all six isoforms of vertebrate actin, globular (G) and fillimentous (F) forms. This clone is a pan-actin antibody that binds to an epitope in a highly conserved region of actin. Labels myotubes and stains myoblasts and fibroblasts. Does not interfere with actin polymerization to form filaments, at a ratio as high as one antibody per two actin monomers. Species Crossreactivity: All vertebrates as well as Dictyostelium discoideum and Physarum polycephalum.
Clone # USB:2Q1055 (C4)
Isotype:IgG2b,k
Calc Applications Abbrev:E IC IF IHC WB
Calc Crossreactivity:Ch Hu Mo Rt
Immunogen:Purified chicken gizzard actin. Epitope: Appears to be specific for the N-terminal two-thirds of the actin molecule, possibly near aa50-70.