The Green Fluorescent Protein (GFP) from the jellyfish Aequorea victoria is used as a fluorescent indicator for monitoring gene expression in a variety of cellular systems, including living organisms and fixed tissues.
Unlike other bioluminescent reporters, GFP fluoresces in the absence of substrates, cofactors, or other intrinsic or extrinsic proteins.
Purified GFP is a 27kD monomer consisting of 238 amino acids and emits green light (emission maximum at 509nm) when excited with blue or UV light.
Applications:Suitable for use in ELISA, Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilutions:ELISA: 1:80,000-1:500,000.
Sandwich or capture for detection of GFP recombinant proteins and enhanced forms of GFP.
Western Blot: 1:3000-1:30,000
Immunohistochemistry (frozen sections.
Not suitable for paraffin sections):1:1000-1:5000
Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：500ug
Host：mouse
Grade：Affinity Purified
Purity：Purified by Protein A affinity chrmoatography.
Form：Supplied as a liquid in PBS, pH 7.2, 0.01% sodium azide
Specificity：Recognized wild type and recombinant forms of GFP. No reaction is seen against RFP. Assay by Immunoelectrophoresis resulted in a single precipitin arc against anti mouse serum.
Clone # USB：1001
Isotype：IgG1,k
Calc Applications Abbrev：E IHC WB
Immunogen：Green Fluorescent Protein (GFP) fusion protein corresponding to the full length amino acid sequence (246aa) derived from the jellyfish Aequorea victoria.
