85-7211-52　Troponin I, Cardiac, aa23-29 (cTnI, CMH7, Familial Hypertrophic Cardiomyopathy 7, MGC116817, TNNC1, Troponin I Type 3 Cardiac, TNNI3) 1mg　T8665-18B

特徴

• Troponin, working in conjunction with tropomyosin, functions as a molecular switch, regulating muscle contraction in response to changes in the intracellular Ca2+ concentration.
• Troponin consists of three subunits, the Ca2+-binding subunit troponin C (TnC), the tropomyosin-binding subunit troponin T (TnT), and the inhibitory subunit troponin I (TnI).
• Troponin I binds to actin in thin myofilaments to hold the actin-tropomyosin complex in place, because of which myosin cannot bind to actin in relaxed muscle.
• When Calcium binds to the Troponin C it causes certain confomational changes which leads to dislocation of troponin I.
• The letter “I” is given due to its inhibitory character.
• The tissue specific subtypes are:Slow-twitch skeletal muscle isoform troponin I, TNNI1 (1q31.3, 191042)
• Fast-twitch skeletal muscle isoform troponin I, TNNI2 (11p15.5, 191043)
• Cardiac troponin I, TNNI3 (19q13.4, 191044)
• Applications:Suitable for use in ELISA, Western Blot and Immunoaffinity Purification.
• Other applications not tested.
• Recommended Dilutions:Optimal dilutions to be determined by the researcher.
• Hybridoma:SP2/0 myeloma cells with spleen cells from Balb/c mice.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.