Cardiac Troponin T, a 36kD protein, is the tropomyosin binding subunit of the troponin complex, which is located on the thin filament of striated muscles and regulates muscle contraction in response to alterations in intracellular calcium ion concentration.
Mutations within the protein have been associated with familial hypertrophic cardiomyopathy as well as with dilated cardiomyopathy.
Transcripts for the gene undergo alternative splicing that results in many tissue specific isoforms.
Troponin, working in conjunction with tropomyosin, functions as a molecular switch, regulating muscle contraction in response to changes in the intracellular Ca2+ concentration.
Troponin consists of three subunits, the Ca2+-binding subunit troponin C (TnC), the tropomyosin-binding subunit troponin T (TnT), and the inhibitory subunit troponin I (TnI).
Troponin I binds to actin in thin myofilaments to hold the actin-tropomyosin complex in place, because of which myosin cannot bind to actin in relaxed muscle.
When Calcium binds to the Troponin C it causes certain confomational changes which leads to dislocation of troponin I.
The letter “I” is given due to its inhibitory character.
Tissue Specific Subtypes:Slow-twitch skeletal muscle isoform troponin I, TNNI1 (1q31.3, 191042)
Fast-twitch skeletal muscle isoform troponin I, TNNI2 (11p15.5, 191043)
Cardiac troponin I, TNNI3 (19q13.4, 191044)
Applications:Suitable for use in ELISA, Western Blotting and Immunoaffinity Purification.
Other applications not tested.
Recommended Dilution:Optimal dilutions to be determined by the researcher.
Hybridoma:Hybridization of Sp 2/0 myeloma cells with spleen cells from Balb/c mice.
Source:Ascites
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：1mg
Host：mouse
Source Antibody：human
Grade：Affinity Purified
Purity：>90%. Purified by chromatography on protein A sepharose. Purity is tested by electrophoresis.
Form：Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Specificity：Reacts with free cardiac Troponon I (cTnl) and cTnl forming complexes with other troponin components. Not affected by heparin, phosphorylation, oxidation and troponin complex formation. Recognizes an epitope located between amino acid residues 186 and 192 of cTnI. Crossreactivity with skeletal troponin I from human, rat and mouse is observed.
