85-7211-40　［取扱停止］Myeloperoxidase (MPO, pANCA) 1mg　M9760-12

特徴

• In normal tissues and in a variety of myeloproliferative disorders, myeloid cells of both neutrophilic and eosinophilic types, at all stages of maturation, exhibit strong cytoplasmic reactivity for MPO.
• It is readily detected in myeloblasts and immature myeloid cells of acute myelogenous leukemia, progranulocytic leukemia, monomyelocytic leukemia, erythro-leukemia, myeloblastomas and other hematopoietic disorders.
• Erythroid precursors, megakaryocytes, lymphoid cells, mast cells and plasma cells are nonreactive.
• Cells of monocytic derivation reveal variable reactivity and are typically weakly positive or nonreactive.
• Cells comprising the infiltrate of a spectrum of lymphoid malignancies, e.g., lymphoblastic lymphoma or leukemia, chronic lymphocytic leukemia, hairy cell leukemia, non-Hodgkin’s lymphomas of T-or B-cell type and Hodgkin’s disease, are nonreactive, as are the non-neoplastic tissues present in these specimens, except for occasional cells of myeloid derivation.
• MPO is not observed in the neoplastic cells of a wide variety of epithelial tumors and sarcomas, or in the contiguous non-neoplastic tissues.
• MPO is useful in differentiating between myeloid and lymphoid leukemias.
• Applications:Suitable for use in ELISA.
• Other applications have not been tested.
• Recommended Dilution:Optimal dilutions to be determined by the researcher.
• Hybridoma:Hybridization of Sp 2/0 myeloma with spleen cells from Balb/c mice.
• Storage and Stability:May be stored at 4°C.
• For long-term storage, aliquot and store at 4°C.
• Do not freeze.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial prior to removing the cap.
• Further dilutions can be made in assay buffer.