Not suitable for use on paraffin embedded sections.
Staining of 10um thick cryostat sections fixed with acetone or ethanol.
Staining is similar to that for polyclonal antibodies to interstitial collagen I.
ELISA: Apparent binding constant from titration curves is approximately 0.1nM.
Suitable for use in sandwich ELISA for specific determination of collagen I, employed as either a capture antibody or as a second, detecting antibody.
Optimal dilutions to be determined by the researcher.
Source:Ascites
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months at -20°C.
Reconstitute with sterile ddH2O.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：mouse
Source Antibody：human
Grade：Highly Purified
Purity：Purified by ammonium sulfate precipitation and chromatography on DEAE-cellulose.
Form：Supplied as a lyophilized powder. No preservative added. Reconstitute with 1ml sterile ddH2O.
Specificity：Recognizes both native and heat denatured human collagen type I. No crossreactivity with albumin fractions of human serum or albumins of other animal species. <5% binding to collagens II and III in ELISA, RIA or immunoblotting.
