ICAM-1 permits antigen-independent adhesion between lymphocytes and their targets and may be used to study cell-cell interactionsor for rhinovirus and malarial studies.
The LFA-1 binding site is located in domain 1 of ICAM-1.
CD54 antigen is a membrane glycoprotein with a wide tissue distribution that includes vascular endothelium and many cells of the immune system.
CD54 is weakly expressed on resting peripheral blood lymphocytes.
Upon activation by mitogens, the CD54 antigen is strongly expressed on B-cells, T-cells, macophages and granulocytes.
ICAM-1 has 7 potential N-linked glycosylation sites.
ICAM-1 is a single chain glycoprotein of Ig supergene family, present on unstimulated endothelial cells and on a variety of other cell types including activated fibroblasts, EC, macrophages and lymphocytes.
ICAM-1 mediates cell adhesion by binding to integrins CD11a/CD18 (leukocyte adhesion molecule, LFA-1) and to CD11b/CD18 (Mac-1).
This interaction enhances antigen-specific T-cell activation.
ICAM-1 also binds to CD43 and to Plasmodium falciparum infected RBCs.
Inhibits adhesion of infected RBCs and LFA-1 to ICAM-1.
CD54 is the receptor for rhinoviruses and malaria.
Applications:Suitable for use in Flow Cytometry, Immunoprecipitation and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Flow Cytometry: 1ug to stain 1x10e6 cells
Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4ºC before opening.
DO NOT FREEZE! Stable at 4ºC as an undiluted liquid.
Dilute only prior to immediate use.
Freezing R-Phycoerythrin (PE) conjugates will result in a substantial loss of activity.
仕様
Size：100ug
Host：rat
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by Protein G affinity chromatography
Form：Supplied as a liquid in PBS, pH 7.2, 1% BSA, 0.09% sodium azide. Labeled with PE.
Specificity：Recognizes the Mr 95kD cell surface glycoprotein known as mouse intracellular adhesion molecule ICAM-1. ICAM-1 is the receptor for LFA-1 (CD11a/CD18) and Mac-1 (CD11b/CD18).
