85-7211-17　［取扱停止］CD54 (Intercellular Adhesion Molecule, ICAM-1) (Biotin) 200ug　C2409-23

特徴

• ICAM-1 permits antigen-independent adhesion between lymphocytes and their targets and may be used to study cell-cell interactionsor for rhinovirus and malarial studies.
• The LFA-1 binding site is located in domain 1 of ICAM-1.
• CD54 antigen is a membrane glycoprotein with a wide tissue distribution that includes vascular endothelium and many cells of the immune system.
• CD54 is weakly expressed on resting peripheral blood lymphocytes.
• Upon activation by mitogens, the CD54 antigen is strongly expressed on B-cells, T-cells, macrophages and granulocytes.
• ICAM-1 has 7 potential N-linked glycosylation sites.
• ICAM-1 is a single chain glycoprotein of Ig supergene family, present on unstimulated endothelial cells and on a variety of other cell types including activated fibroblasts, EC, macrophages and lymphocytes.
• ICAM-1 mediates cell adhesion by binding to integrins CD11a/CD18 (leukocyte adhesion molecule, LFA-1) and to CD11b/CD18 (Mac-1).
• This interaction enhances antigen-specific T-cell activation.
• ICAM-1 also binds to CD43 and to Plasmodium falciparum infected RBCs.
• Inhibits adhesion of infected RBCs and LFA-1 to ICAM-1.
• CD54 is the receptor for rhinoviruses and malaria.
• Application(s): Anti-CD54 (ICAM-1) may be used to study cell-cell interactions.
• Recommended Dilution: We recommend using 1ug to stain 1 x 10e6 cells in flow cytometric applications.
• Storage and Stability:Store product at 4°C if to be used immediately within two weeks.
• For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C.
• Aliquots are stable at -20°C for 12 months after receipt.
• Dilute required amount only prior to immediate use.
• Further dilutions can be made in assay buffer.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.