CD41 is the integrin alpha IIb chain, also called gpIIb, which is non-covalently associated with the integrin beta 3 chain, also called gpIIIa (CD61), forming the gpIIbIIIa complex.
Antibody reacts specifically with normal platelets and megakaryocytes but studies have shown that it does not react with Glanzmann’s thrombasthenic platelets which lack the membrane glycoprotein gpllbllla.
Does not inhibit the platelet aggregation and secretion induced by collagen, arachidonic acid and thrombin.
Does not inhibit the fibrinogen binding to human platelets induced by ADP, arachidonic acid and PAF.
The CD41 antigen is present on virtually all human platelets and is also found on megakaryocytes
Applications:Suitable for use in Flow Cytometry.
Other applications have not been tested.
Recommended Dilutions:
Flow Cytometry: Use 1ug to stain 1.0 x 10e6 cells.
Optimal dilutions to be determined by the researcher
Storage and Stability:Store product at 4°C if to be used immediately within two weeks.
For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C.
Aliquots are stable at -20°C for 12 months after receipt.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ug
Host：mouse
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein G affinity chromatography from cell culture
Form：Supplied as a liquid in PBS, pH 7.4, 1% BSA, 0.09% sodium azide. Labeled with Biotin.
Specificity：Recognizes an epitope on the gpIIb/IIIa complex on the surface membrane of human platelets. Precipitates two bands under non-reducing conditions (Mr 110kD and 130kD).
