The beta-lactam antibiotics (penicillins and cephalosporins) are the most frequently used antimicrobial agents.
All of the beta-lactams are structurally related through the presence of a core beta-lactam ring.
Bacterial resistance to beta-lactams continues to increase, primarily due to the production of beta-lactamases.
Beta-lactamases catalyze the hydrolysis of the beta-lactam bond which destroys antibacterial activity.
Bacteria that produce TEM-or SHV-type beta-lactamases have point mutations in structural genes that have extended the substrate specificity of these beta-lactamases.
As a result, many of the beta-lactamase-producing Gram-negative pathogens have become multi-drug resistant.
Applications:Suitable for use in ELISA and Western Blot.
Other applications not tested.
Recommended Dilutions:ELISA: 10-20ug/ml
Western Blot: 10ug/ml
Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：500ug
Host：mouse
Grade：Affinity Purified
Purity：Purified by Protein G affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4. No preservatives added.
Specificity：Recognizes TEM-type beta-lactamases.
Clone # USB：1.BB.875 (8A5.A10)
Isotype：IgG1
Calc Applications Abbrev：E WB
Immunogen：Recombinant 5'-His-tagged TEM-1 beta-lactamase derived from E. coli 205 TEM.
