L1 Protein or Calprotectin Molecule is an intracytoplasmic antigen comprised of a 12kD alpha chain and a 14kD beta chain.
The antigen is expressed by granulocytes, monocytes and by tissue macrophages.
Applications:Suitable for use in Immunohistochemistry.
Other applications have not been tested.
Recommended Dilutions:Immunohistochemistry (Formalin/paraffin): 1:1000 for 20 minutes at RT.
For staining of formalin-fixed tissues, digest sections with trypsin at 1mg/ml PBS for 10 minutes at 37°C.
Optimal dilutions to be determined by the researcher.
Recommended Positive Control:Tonsil, lymph node or spleen
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：mouse
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein G affinity chromatography from ascites.
Form：Supplied as a liquid in 10mM PBS, pH 7.4. No preservatives added. No stabilizing proteins added. Also available with BSA and azide. See M1202-74.
Specificity：Recognizes human Macrophage. Reacts with neutrophils, monocytes and squamous mucosal epithelia. Has been shown as an excellent marker in tissue sections. Species Crossreactivity: baboon, monkey, porcine, feline, canine, guinea pig, rat and mouse.
