The gp100 protein is the most abundant glycoprotein in nucleated mammalian cells.
The gp100 antigen is highly expressed in melanocyte cells, and recognized by cytotoxic T lynphocytes.
The gp100 protein is known to initiate cellular immune response through the interaction with mature dendritic cells (DCs).
GP100/PMel17 is a melanoma differentiation antigen, which is observed as the target of tumor infiltrating lymphocytes (TIL) and has therefore been proposed as an
immunotherapeutic agent in malignant melanoma.
Applications:Suitable for use in Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Immunohistochemistry (Immunoperoxidase staining on frozen tissue sections): 1:5-1:20 with PBS.
Formalin fixation and embedding in paraffin may affect the reactivity of the antibody.
Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：mouse
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein G affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.1% BSA, 0.02% sodium azide. Also available without BSA and Azide. See M2869-01X.
Specificity：Recognizes a human (pre)melanosomal 100+7kD antigen (glycoprotein). Reacts with melanomas, clear cells sarcomas (melanoma of soft tissue), nevocellular nevi and normal melanocytes. Except for one case of non-Hodgkin’s lymphoma in which macrophages were positive, no reactions with other tumors or tissues have been observed.
