MUC16/CA125 antigen is a serum marker that is used routinely in gynecologic practice to monitor patients with ovarian cancer.
It is a mullerian duct differentiation antigen that is overexpressed in epithelial ovarian cancer cells and secreted into the blood, although its expression is not entirely confined to ovarian cancer.
Most biochemical studies have concluded that MUC16/CA125 is a high molecular mass glycoprotein.
According to the ISOBM-TD-1 workshop, CA125 contains three antigenic domains: A-domain (Oc125), B-domain (M11), C-Domain (Ov197).
The CA-125 antigen is present on a mucin-like glycoprotein of >200kD and is detectable on the apical surface of normal endometrium and endocervix.
Applications:Suitable for use in ELISA and Western Blot.
Other applications not tested.
Recommended Dilutions:Optimal dilutions to be determined by the researcher.
Matched Pair:Suitable for use as the capture or solid phase antibody.
Matched to C0050-13 as the detection or conjugate (label) antibody.
Hybridoma:Sp2/0 myeloma cells with spleen cells from Balb/c mice.
Source: Ascites
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：1mg
Host：mouse
Source Antibody：human
Grade：Highly Purified
Purity：Purified by Ion Exchange chromatography. >90% Purity is tested by electrophoresis.
Form：Supplied as a liquid in 10mM Tris, 0.15M sodium chloride, pH 7.5, 0.05% sodium azide.
Specificity：Recognizes B-domain in the protein core of the CA125 ovarian cancer antigen.
Clone # USB：1.B.829
Isotype：IgG1
Calc Applications Abbrev：E WB
Calc Crossreactivity：Hu
Immunogen：CA125 antigen purified from human ovarian carcinoma (MW >1 million Daltons).
