85-7210-58　Eosinophil Major Basic Protein 100ug　E3330

特徴

• Applications:Suitable for use in Immunohistochemistry, Western Blot and ELISA.
• Other applications have not been tested.
• Recommended Dilutions:Immunohistochemistry: Reacts with fresh frozen tissues that were sectioned (6um), dried for 1 hour at RT and fixed in 1:1 solution of methanol and acetone, and airdried for 1 hour.
• Sections can be stored wrapped in aluminium foil at -70°C until used.
• Immunohistochemistry (FFPE): 4% PFA (3hr) should be used for skin biopsies.
• 5uM sections are dewaxed, and rehydrated with water.
• A proteinase treatment of 0.1% trypsin for 15 minutes in water should be performed prior to blocking and antibody staining.The antibody reacts better in frozen tissues than in paraffin embedded tissues.
• Western Blot: Purified normal human eosinophils were treated with digitonin (5mg/ml) for 10 minutes before boiling in sample buffer and run on a 15% SDS-PAGE.
• Purified MBP migrates at ~ 14kDa, and typical human samples range from 10-16kDa.
• Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.