b2-microglobulin non-covalently associates with the 44kD a-chain to form the HLA Class I antigen complex.
Human b2-microglobulin associated with HLA Class I antigens is expressed on many types of cells including lymphocytes, thymocytes, monocytes, granulocytes, platelets, endothelial cells, and epithelial cells.
It is absent on erythrocytes.
Applications:Suitable for use in Immunofluorescence, Flow Cytometry and Immunohistochemistry.
Immunohistochemistry (Frozen sections or cell smears): 1:25-1:50
Optimal dilutions to be determined by the researcher.
Hybridoma:NS1/Ag4.1 myeloma cells with spleen cells of Balb/c mice.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months after receipt at -20°C.
Reconstitute with sterile ddH2O.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：200ug
Host：mouse
Source Antibody：human
Grade：Purified
Purity：Purified
Form：Supplied as a lyophilized powder from PBS, 1mg/ml BSA. Reconstitute with 1ml sterile ddH2O.
Specificity：Recognizes human Beta 2-microglobulin (MW 12kD). Does not react with Beta 2-microglobulin from other species. Recognizes the HLA-Beta 2-microglobulin complex, free or associated with the membrane. The exact epitope for the antibody has not been mapped.
