Human D-dimer (DD), a specific degradation product of cross-linked fibrin, is released into the bloodstream during clot breakdown.
It is used as an indicator of a blood clots, venous thromboses or pulmonary embolisms.
Applications:Suitable for use in ELISA and Western Blot.
Other applications have not been tested.
Recommended Dilution:Sandwich ELISA: Plasma samples should be diluted with 10mM Tris-HCl buffer, pH 7.5, 1M sodium chloride, 0.1% Tween 20.
To avoid non-specific binding, the final sodium chloride concentration in plasma samples should be ≥ 0.5M.
Western Blot: Non-reducing
Optimal dilutions to be determined by the researcher.
Matched Pair:Suitable for use as the capture or solid phase antibody.
Matched to F4197-17C or F4197-17D, F4197-15 as the detection or conjugate (label) antibody in a 1-step sandwich immunoassay.
Matched to F4197-17, F4197-18 and F4197-20, F4197-17D, F4197-17C as the detection or conjugate (label) antibody in a 2-step
sandwich immunoassay.
Hydridoma:Hybridization of Sp2/0 myeloma cells with spleen cells from Balb/c mice.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：1mg
Host：mouse
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A sepharose chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide.
Specificity：Recognizes human D-dimer (DD), a specific degradation product of cross-linked fibrin, as well as a high molecular weight fibrin degradation products. Does not crossreact with D-monomer and fibrinogen.
