S100 belongs to the family of calcium binding proteins such as calmodulin and troponin C.
S100A is composed of an alpha and beta chain whereas S100B is composed of two beta chains.
Antibody to S100 stains Schwannomas, ependymomas, astrogliomas, almost all benign and malignant melanomas and their metastases.
S100 protein is also expressed in the antigen presenting cells such as the Langerhans cells in skin and interdigitating reticulum cells in the paracortex of lymph nodes.
S100 is a nervous tissue specific soluble protein made in glial cells.
Present in sweat glands chongrocytes and myoepithelial cells.
May be found as cytoplasmic, membranous or mixed throughout the areas of tumor.
Usually cytoplasmic when present in normal tissues.
Applications:Suitable for use in Western Blot, ELISA and Immunoaffinity Purification.
Other applications not tested
Recommended Dilutions:Optimal dilutions to be determined by researcher.
Matched Pairs:Capture: S0052-11, S0052-15
Detection: S0052-14
Hybridoma:Sp2/0 myeloma cells with spleen cells from Balb/c mice.
Source: Ascites
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：1mg
Host：mouse
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A affinity chromatography (≥ 90%). Purity tested by electrophoresis.
Form：Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide.
Specificity：Recognizes S100 (beta, beta) and S100 (alpha, beta) protein.
