Enolases are homo- or heterodimers of the three subunits: alpha, 46kD, beta, 44kD, and gamma, 46kD.
The alpha-subunit is expressed in most tissues and the beta-subunit only in muscle.
The gamma subunit is expressed primarily in neurons, in normal and in neoplastic neuroendocrine cells.
Coexpression of NSE and chromogranin A is common in neuroendocrine neoplasms.
Applications:Suitable for use in ELISA and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Immunohistochemistry: 1:100-1:300 (Formalin-fixed and paraffin-embedded sections).
Optimal dilutions to be determined by the researcher.
Matched Pair for ELISA:Capture: Detection:N2175-09 N2175-06
N2175-06 N2175-09
Affinity Constant: 1x10e-8 to 1x10e-9
Hybridoma:Sp2/0 myeloma cells with spleen cells from Balb/c mice.
Source: Ascites
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20°C or colder.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：1mg
Host：mouse
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein G affinity chromatography. Purity is tested by electrophoresis.
Form：Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide.
Specificity：Specific for human gamma enolase subunit. No detectable crossreactivity with the alpha or beta subunits. Reacts with 45kD band corresponding to NSE in Immunoblot of human brain extracts.
Clone # USB：1.B.652
Isotype：IgG2b
Calc Applications Abbrev：E IHC
Calc Crossreactivity：Hu
Immunogen：Purified neuron-specific enolase from human brain.
