Enolases are homo- or heterodimers of the three subunits: alpha (46kD), beta (44kD) and gamma (46kD).
The alpha-subunit is expressed in most tissues.
The beta-subunit is expressed only in muscle.
The gamma subunit is expressed primarily in neurons, in normal and in neoplastic neuroendocrine cells.
Coexpression of NSE and chromogranin A is common in neuroendocrine neoplasms.
Applications:Suitable for use in ELISA and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Immunohistochemistry: Formalin-fixed and paraffifn-embedded sections.
Optimal dilutions to be determined by the researcher.
Matched Pairs:Capture: N2175-06, N2175-07
Detection: N2175-07, N2175-06
Hybridoma:Sp2/0 myeloma cells with spleen cells from Balb/c mice.
Source: Ascites
Affinity Constant:1x10e-8 to 1x10e-9
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：1mg
Host：mouse
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein G affinity chromatography (≥ 95%). Purity is tested by electrophoresis.
Form：Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide.
Specificity：Gamma enolase. Specific for the gamma subunit without detectable crossreactivity with the alpha or beta subunits. Reacts with 45kD band corresponding to NSE in immunoblotting of human brain extracts.
Clone # USB：1.B.650
Isotype：IgG2a
Calc Applications Abbrev：E IHC
Calc Crossreactivity：Hu
Immunogen：Purified neuron-specific enolase from human brain.
