Applications:Suitable for use in ELISA, Dot Blot and Immunofluorescence.
Other applications not tested.
Recommended Dilutions:Optimal dilutions to be determined by the researcher.
Affinity Constant:LPS Group A: 2.1 x 10e9L/mole
LPS Group B: 1.1 x 10e9L/mole
LPS Group D: 6.5 x 10e8L/mole
LPS Group E: 1.0 x 10e6L/mole
Source: Ascites
Hybridoma:Myeloma Sp2/0 cells with spleen cells of Balb/c mice.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size:500ug
Host:mouse
Grade:Affinity Purified
Purity:Purified by Protein G affinity chromotography from ascites.
Form:Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide.
Specificity:Recognizes Antigen Determinant 0-12 of Salmonella O-antigens (A, B and D group). Strain Crossreactivity: Salmonella paratyphi, Salmonella typhimurium, Salmonella enteriditis, Salmonella anatum and Salmonella selandia. Does not crossreact with: Salmonella cholerase, Salmonella newport, E. coli O55:B5, E. coli K12 and Klebsiella pneumonia
Clone # USB:1.B.480
Isotype:IgG1
Calc Applications Abbrev:DB E IF
Immunogen:Heat inactivated bacterial cells of Salmonella paratyphi (Group A), Salmonella typhimurium (Group B) and Salmonella enteriditis (Group D).