Hepatitis C virus (HCV) represents the major etiologic agent of non-A, non-B hepatitis.
The pathological changes in hepatitis C are characterized by the histologic triad of lymphoid aggregates in portal tracts, epithelial damage of small bile ducts and micro-and macro-vesicular steatosis.
HCV antigens are visible in the cytoplasm of infected hepatocytes by immunohistochemical staining with mono-or polyclonal antibodies.
These studies are important to determine the viral load and expression pattern of HCV in the infected liver.
The mechanism of hepatocyte and bile duct injury in HCV infection appears to be related to the host immune response to the virus.
Direct viral cytopathogenicity may also play a role.
Applications:Suitable for use in Western Blot, FLISA, Immunofluorescence and Immunohistochemistry.
Other applications not tested.
Recommended Dilutions:Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
FITC conjugates are sensitive to light.
仕様
Size：100ug
Host：mouse
Grade：Affinity Purified
Purity：Purified by Protein A affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2, 0.01% sodium azide. Labeled with Fluorescein isothiocyanate (FITC)
Specificity：Recognizes HCV NS4 antigen at ~19kD.
Clone # USB：1.B.356
Isotype：IgG2a
Calc Applications Abbrev：FLISA IF IHC WB
Immunogen：HCV NS4 recombinant antigen (NS4a+b) corresponding to aa1658-1863
