Hepatitis C virus (HCV) represents the major etiologic agent of non-A, non-B hepatitis.
The pathological changes in hepatitis C are characterized by the histologic triad of lymphoid aggregates in portal tracts, epithelial damage of small bile ducts and micro-and macro-vesicular steatosis.
HCV antigens are visible in the cytoplasm of infected hepatocytes by immunohistochemical staining with mono-or polyclonal antibodies.
These studies are important to determine the viral load and expression pattern of HCV in the infected liver.
The mechanism of hepatocyte and bile duct injury in HCV infection appears to be related to the host immune response to the virus.
Direct viral cytopathogenicity may also play a role.
Applications:Suitable for use in ELISA and Immunohistochemistry.
Optimal dilutions to be determined by the researcher.
Positive Control:H1920-04: Hepatitis C Virus Core, 22kD, aa2-192, Recombinant (HCV)
Storage and Stability:Store product at 4°C if to be used immediately within two weeks.
For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C.
Aliquots are stable at -20°C for 12 months after receipt.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：mouse
Grade：Affinity Purified
Purity：Purified by Protein A affinity chromatography from cell culture.
Form：Supplied as a liquid in PBS, pH 7.2, 0.01% sodium azide. Labeled with Biotin.
Specificity：Recognizes HCV core antigen of live virus, lysates and recombinant antigen. Reactive with genotypes 1a, 1b, 2a and 2b. Epitope: aa1-80 of HCV core antigen.
Clone # USB：1.B.345
Isotype：IgG2a
Calc Applications Abbrev：E IHC
Immunogen：Recombinant protein corresponding to HCV.
