Cholera toxin is comprised of two subunits, A and B.
Subunit A is an ADP-ribosyltransferase, which disrupts the proper signaling of G proteins, leading to cell dehydration.
The 12kD nontoxic subunit B ("choleragenoid"), which is assembled into a 60kD pentamer above pH 2, allows the protein complex to bind to cellular surfaces via the pentasaccharide chain of ganglioside GM1.
Applications:Suitable for use in ELISA.
Other applications have not been tested.
Recommended Dilution:ELISA: 1:10e-3
Optimal dilutions to be determined by the researcher.
Hybridoma:SP2/0 myeloma cells with spleen cells from Balb/c mice.
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage, store at -20°C.
Aliquots are stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：1mg
Host：mouse
Grade：Affinity Purified
Purity：>90%. Purified by Protein A Sepharose chromatography. Purity is tested by electrophoresis.
Form：Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide and 40% glycerol.
Specificity：Beta-subunit of chorera toxin. Reacts with cholera toxin and enterotoxin of E. coli.
