85-7209-71　［取扱停止］Chlamydia trachomatis MOMP (Major Outer Membrane Protein) 1ml　C4250-62

特徴

• Chlamydia is caused by the bacterium Chlamydia trachomatis.
• The intracytoplasmic inclusions caused by the bacterium are draped around the infected cell’s nucleus.
• Chlamydia has a genome size of approximately 500-1000 kilobases and contains both RNA and DNA.
• The organism is also extremely temperature sensitive and must be refrigerated at 4°C as soon as a sample is obtained.
• Colonization of Chlamydia begins with attachment to sialic acid receptors on the eye, throat or genitalia.
• It persists at body sites that are inaccessible to phagocytes, T-cells, and B-cells.
• It also exists as 15 different serotypes.
• These serotypes cause four major diseases in humans: endemic trachoma (caused by serotypes A and C), sexually transmitted disease and inclusion conjunctivitis (caused by serotypes D and K), and lymphogranuloma venereum (caused by serotypes L1, L2, and L3).
• Studies reveal that Chlamydia, because of its cell wall, is able to inhibit phagolysosome fusion in phagocytes.
• The cell wall is proposed to be gram-negative in that it contains an outer lipopolysaccharide membrane, but it lacks peptidoglycan in its cell wall.
• This lack of peptidoglycan is shown by the inability to detect muramic acid and antibodies directed against it.
• It may, however, contain a carboxylated sugar other than muramic acid.
• The proposed structure consists of a major outer membrane protein cross-linked with disulfide bonds.
• It also contain cysteine-rich proteins (CRP) that may be the functional equivalent to peptidoglycan.
• This unique structure allows for intracellular division and extracellular survival (Hatch 1996).
• -Kenneth Todar, University of Wisconsin-Madison
• Applications:Suitable for use in ELISA.
• Other applications not tested.
• Recommended Dilution:Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for at least 12 months.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.