PAPP A (PAPP-A, Insulin-like growth factor binding protein-4 protease, PAPP-A-proMBP complex) was originally isolated from pregnancy serum as heterotetrameric protein complex with molecular weight approximately 500kD, consisting of two PAPP A subunits disulfide-linked with two subunits of the proform of eosinophil major basic protein (proMBP).
In such a complex proMBP functions as an inhibitor of PAPP A proteinase activity.
PAPP A is widely used as a marker of some pathologies during pregnancy.
Low maternal serum levels of PAPP-A in first trimester biochemical screening is used as a marker of Down’s syndrome (Trisomy 21).
Applications:Suitable for use in ELISA.
Other applications have not been tested.
Recommended Dilution:Optimal dilutions to be determined by the researcher.
Matched Pairs for detection of htPAPP-A in Sandwich Immunoassay:Capture: P6000-80 Detection: P6000-89
Matched Pairs for detection of htPAPP-A and dPAPP-A in Sandwich Immunoassay:Capture: P6000-82 Detection: P6000-80
P6000-80 P6000-80A
Hybridoma:Hybridization of SP 2/0 myeloma cells with spleen cells from Balb/c mice.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ug
Host：mouse
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A affintiy chromotography. Purity is tested by SDS-PAGE, ~90%.
Form：Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide. Also availible without azide. See P6000-80U.
Specificity：Recognizes human PAPP-A antigen.
Clone # USB：1.B.130
Isotype：IgG2b
Calc Applications Abbrev：E
Calc Crossreactivity：Hu
Immunogen：Purified human PAPP-A /pro MBP heterotetrameric complex (htPAPP-A)
