85-7209-25　CUG-Binding Protein 1 Ribonucleoprotein (CUG-BPI, Cytidine Uridine Guanosine Binding Protein 1) 200ug　C7948-50

特徴

• Myotonic dystrophy (MD) is an autosomal dominant neuromuscular disease that is associated with a (CTG)n repeat expansion in the 3'-untranslated region of the myotonin protein kinase (Mt-PK) gene.
• A (CUG)n oligonucleotides triplet repeat pre-mRNA/mRNA binding protein may play an important role in DM pathogenesis.
• HeLa cell protein, CUG-BP1, has been purified based upon its ability to bind specifically to (CUG)8 oligonucleotides in vitro.
• CUG-BP1 is the major (CUG)8 binding activity in normal cells.
• CUG-BP1 has been identified as isoforms of a novel heterogeneous nuclear ribonucleoprotein (hnRNP), hNab50.
• The CUG-BP/hNab50 protein is localized predominantly in the nucleus and is associated with polyadenylated RNAs in vivo.
• In vitro RNA-binding/photocrosslinking studies demonstrate that CUG-BP/hNab50 binds to RNAs containing the Mt-PK 3'-UTR.
• Applications:Suitable for use in Western Blot, Immunoprecipitation, Immunocytochemistry and Supershift Assay.
• Other applications have not been tested.
• Recommended Dilutions:Western Blot: 0.1-2.0ug/ml detects CUG-BP1 in HeLa nuclear extract.
• HeLa nuclear extract was resolved by electrophoresis, transferred to nitrocellulose and probed with C7948-50 (0.5ug/ml).
• Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system.
• Immunoprecipitation: Immunoprecipitates CUG-BP1 from HeLa cell lysates
• Immunocytochemistry: Nuclear staining in HeLa and myoblasts from normal patients.
• Supershift: Supershifts CUG-BP1:DNA complexes in EMSA
• Optimal dilutions to be determined by the researcher.
• Recommended secondary antibodies:I1904-06C: IgG, H&L (HRP) (X-Adsorbed) Pab Gt xMo I1904-06H IgG, H&L (HRP) (X-Adsorbed) Pab Gt xMo I1904-14C: IgG, Fc (HRP) Pab Gt xMo
• I1904-19C: IgG, F(ab’)2 (HRP) Pab Gt xMo
• Included Positive Antigen Control:H1838: HeLa nuclear extract.
• Add an equal volume of Laemmli reducing sample buffer to 5-10ul of extract and boil for 5 minutes to reduce the preparation.
• Load 20ug of reduced lysate per lane for minigels.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.