Alpha-1-antitrypsin (AAT) consisting of a single polypeptide chain and has a molecular weight of 51kD.
It exists in a number of genetic variants.
MM variant is the most common.
AAT is synthesized in the liver and it acts as an inhibitor of proteases such as trypsin, elastase, chymotrypsin, collagenase, leukocytic proteases, plasmin and thrombin, which may be released during inflammatory reactions in the lung.
In the absence of AAT, these enzymes are not inhibited and they may digest pulmonary parenchyma.
AAT deficiency is associated with chronic obstructive lung disease (emphysema) and less frequently with hepatic cirrhosis in infants and respiratory distress of the newborn.
Increase in AAT occurs as an acute phase response to tissue necrosis and inflammation.
Serum level of AAT is elevated in rheumatoid arthritis, bacterial infections, vasculitis and carcinomatosis.
In colorectal and lung carcinomas, AAT has also related to their invasive and metastatic capacity.
The average level of AAT in plasma is 1.3g/L.
Applications:Suitable for use in ELISA.
Other applications have not been tested.
Recommended Dilutions:Optimal dilutions to be determined by the researcher.
Hybridoma:P3X63-Ag8.653 myeloma cells with spleen cells from Balb/c mice.
Affinity Constant:2x10e9L/mole
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：1mg
Host：mouse
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A affinity chromatography from ascites.
Form：Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide.
Specificity：Recognizes human Alpha-1-Antitrypsin (AAT). No crossreactivity with alpha-1-acid glycoprotein, serum albumin or other serum proteins.
