PDGFs are disulfide-linked dimers consisting of two 12.0-13.5kD polypeptide chains, designated PDGF-A and PDGF-B chains.
The three naturally occurring PDGFs.
(PDGF-AA, PDGF-BB and PDGF-AB) are potent mitogens for a variety of cell types including smooth muscle cells, connective tissue cells, bone and cartilage cells, and some blood cells.
The PDGFs are stored in platelet alpha-granules and are released upon platelet activation.
The PDGFs are involved in a number of biological processes, including hyperplasia, chemotaxis, embryonic neuron development, and respiratory tubule epithelial cell development.
Two distinct signaling receptors used by PDGFs have been identified and named PDGFR-alpha and PDGFR-beta.
PDGFR-alpha is high-affinity receptor for each of the three PDGF forms.
On the other hand, PDGFR-beta interacts with only PDGF-BB and PDGF-AB.
PDGF is released when platelets adhere to traumatized tissues.
Connective tissue cells near the traumatized region respond by initiating the process of replication.The synthesis of PDGF can be induced by Interleukin-1, Interleukin-6, TNF-alpha, TGF-beta and EGF.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20°C or colder.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
Also Available:P4258: Platelet Derived Growth Factor AA, Recombinant, Human (PDGF-AA)
P4258-01: Platelet Derived Growth Factor AA, Recombinant, Human (PDGF-AA)
P4258-01A: Platelet Derived Growth Factor AA, Recombinant, Human (PDGF-AA)
P4258-03: Platelet Derived Growth Factor AA, Recombinant, Human (PDGF-AA) Mab Mo xHu
P4258-04: Platelet Derived Growth Factor AA (PDGF-AA) Pab Rb xHu
Purity：Purified by Protein G affinity chromatography.
Form：Supplied as a liquid in 0.1M Tris HCl, pH 7.5.
Specificity：Recognizes recombinant PDGF-AA with variable intensity depending upon the reduced state of the polypeptide as evidenced by Western analysis.
