Macrophage inflammatory protein-1 alpha (MIP-1 alpha) belongs to the intercrine beta (chemokine CC) family.
MIP-1 alpha is involved in the acute inflammatory state, in the recruitment and activation of polymorphonuclear leukocytes and can inhibit infection of cells with humanimmunodeficiency virus type 1 (HIV-1).
MIP-1 alpha also acts as a potent growth, survival, and chemotactic factor in multiple melanoma cells.
Two know isoforms of MIP-1 alpha can be found; LD78 alpha and LD78 beta.
Depending on the isoform or its N-terminal processing, one or more of the CC chemokine receptors CCR1, CCR2, CCR3 and CCR5 can recognize MIP alpha, thus inhibiting HIV infection.
Applications:Suitable for use in ELISA and Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 17ug/ml using an alkaline phosphatase detection system.
Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：mouse
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein G affinity chromatography.
Form：Supplied as a liquid in 0.1M Tris-HCl, pH 7.5.
Specificity：Recognizes native and recombinant rhMIP-1a and rhMIP-1b. Does not recognize rhMIP-2a or rhMIP-2b.
Clone # USB：1.A.10
Isotype：IgG1
Calc Applications Abbrev：E WB
Calc Crossreactivity：Hu
Immunogen：Purified human recombinant MIP-1a produced in genetically engineered yeast.
