The superfamily of insulin-like growth factor (IGF) binding proteins include the six high-affinity IGF binding proteins (IGFBP) and several low-affinity binding proteins referred to as IGFBP related proteins (IGFBP-rP).
All IGFBP superfamily members are cysteine-rich proteins with conserved cysteine residues, which are clustered in the amino- and carboxy-terminal thirds of the molecule.
IGFBPs modulate the biological activities of IGF proteins.
Some IGFBPs may also have intrinsic bioactivity that is independent of their ability to bind IGF proteins.
Post-translational modifications of IGFBP, including glycosylation, phosphorylation and proteolysis, have been shown to modify the affinities of the binding proteins to IGF.
Applications:Suitable for use in ELISA and Western Blot.
Other applications not tested.
Recommended Dilutions:Western Blot: 1:100; beta-mercaptoethanol should be used as the reducing agent for SDS-PAGE prior to blotting.
Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：mouse
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein G affinity chromatography.
Form：Supplied as a liquid in 0.1M Tris HCl, pH 7.5.
Specificity：Recognizes human IGFBP-4. Does not crossreact with other IGF-BP’s.
Clone # USB：1.A.17
Isotype：IgG1
Calc Applications Abbrev：E WB
Calc Crossreactivity：Hu
Immunogen：Human recombinant IGF-binding Protein 4 expressed in yeast.
