Mast cells contain a number of preformed chemical mediators such as histamine, chymase, carboxypeptidase and proteolytic tryptase.
A substantial quantity of tryptase is estimated to be found in mast cells of skin and lung and suggestes this enzyme plays a major role in mast cell mediated events.
In vitro studies indicate tryptase can cleave C3 to form C3a anaphylatoxin, inactivate fibrinogen as a coaguable substrate for thrombin and activate latent collagenase.
Human mast cell tryptase has considerable potential as a marker for mast cell activation as well as being a mediator of inflammation.
Applications:Suitable for use in Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:50 for 2hrs at RT.
Immunohistochemistry (Formalin/paraffin): 1:2000 for 30 min at RT.
Staining of formalin/paraffin tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.
Optimal dilutions to be determined by the researcher.
Hybridoma:Produced by fusion between BALB/c splenocytes and myeloma NS1 cells.
Positive Control:Tonsil
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：mouse
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein G affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4. Also available with BSA and sodium azide. See M2415-01.
Specificity：Recognizes human Mast Cell Tryptase. Reacts with Mast Cell Tryptase, both a and b isoforms. Species Crossreactivity: Monkey, canine and feline.
Clone # USB：1.N.5
Isotype：IgG1
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Ca Fe Hu Mk
Immunogen：Human mast cell tryptase isolated from purified human lung tissuesCellular Localization: Cytoplasmic
