VDR is a member of the super family of steroid hormone receptors.
It is known to play a role in cellular growth and differentiation, calcium and phosphorus metabolism and bone remodeling.
Human breast carcinomas, leukemias, and prostatic carcinoma cells express VDR, which can modify their growth and differentiation.
Human breast carcinomas, leukemias, and prostatic carcinoma cells express VDR which can modify their growth and differentiation.
Applications:Suitable for use in Gel Supershift Assay and Western Blot.
Other applications have not tested.
Recommended Dilution:Gel Supershift Assay: 1mg/ml
Western Blot: 1-2ug/ml at room temperature for 2 hours.
Optimal dilutions to be determined by the researcher.
Positive Control:MCF-7 cells
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rat
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein G affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4. Also available with BSA and azide. See V2130.
Specificity：Recognizes both the occupied and unoccupied forms of VDR. Epitope: aa89-105 near the DNA-binding domain of human VDR. Does not crossreact with estrogen and glucocorticoid receptors. In mammals, recognizes a protein of ~53kD which is identified as vitamin D Receptor (VDR). In avians, VDR appears as a doublet of 58 and 60kD. Species Crossreactivity: Human, porcine, mouse, rat, hamster, frog, chicken and fish.
Clone # USB：0.N.603
Isotype：IgG2b
Calc Applications Abbrev：WB
Calc Crossreactivity：Av Ch Fr Hm Hu Mo Po Rt
Immunogen：Vitamin D receptor protein from chicken intestine (53kD). Cellular Localization: Nuclear.
