Tyrosinase is a copper-containing metalloglycoprotein that catalyzes several steps in the melanin pigment biosynthetic pathway; the hydroxylation of tyrosine to L-3,4-dihydroxy- phenylalanine (dopa), and the subsequent oxidation of dopa to dopaquinone.
Mutations of the tyrosinase gene occur in various forms of albinism.
Tyrosinase is one of the targets for cytotoxic T-cell recognition in melanoma patients.
Staining of melanomas with antibody showed tyrosinase in melanotic as well as amelanotic variants (1).
This antibody is a useful marker for melanocytes and melanomas.
Applications:Suitable for use in Immunocytochemistry, Western Blot, Immunofluorescence and Immunohistochemistry.
Other applications have not been tested.
Recommended Dilutions:Western Blot: 1:25-1:100 for 2 hours at RT.
Immunohistochemistry (formalin/paraffin): 1:50-1:100 for 30 minutes at RT.
Staining of formalin-fixed tissues requires boiling tissue sections in 1mM EDTA, pH 8.0, for 10-20 minutes followed by cooling at RT for 20 minutes.
Optimal dilutions to be determined by the researcher.
Positive Control:Melanoma cell lines and Melanoma
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：mouse
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4. No preservatives or stabilizing proteins added. Also available with BSA and azide. See T9230-05.
Specificity：Recognizes a cluster of proteins between 70-80kD, identified as human tyrosinase. Occasionally a minor band at 55kD is also detected. Does not crossreact with MAGE-1 and tyrosinase-related protein 1, TRP-1/gp75. Species Crossreactivity: canine and feline. Weak crossreactivity with mouse.
