Microtubules, the major cytoskeletal elements found in all eukaryotic cells, consist of Tubulin, which is a dimer of two 55kD subunits: alpha and Beta.
Microtubules play key roles in chromosome segregation in mitosis, intracellular transport, ciliary and flagellar bending, and structural support of the cytoskeleton.
Applications:Suitable for use in Electron Microscopy, Immunofluorescence, Immunoprecipitation, Western Blot and Immunohistochemistry.
Other applications have not been tested.
Recommended Dilutions:Western Blot: 1-2ug/ml for 2hrs at RT
Immunohistochemistry (Formalin/paraffin): 1:400 for 20 minutes at RT.
No special pretreatment is requiredfor stainingof formalin-fixed, paraffin-embedded tissues.
Immunoprecipitation (Native and denatured): 2ug/mg protein lysate using Protein G.
Optimal dilutions to be determined by the researcher.
Positive Controls:LS174T, MAD109 cells, skin and lung
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：mouse
Source Antibody：chicken
Grade：Affinity Purified
Purity：Purified by Protein G affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4. No stabilizing proteins or preservatives added. Also available with BSA and azide. See T9155-50.
Specificity：Recognizes chicken tubulin alpha at ~57kD. Causes the 10nm filaments to collapse into large lateral aggregates collecting in the cell periphery or tight juxtanuclear caps (1). Does not block microtubule assembly (1). Does not inhibit polymerization or depolymerization of platelet tubulin in vitro (3). Blocks (70-80%) the ability of tubulin dimers (with GppNHp bound) to promote a stable inhibition of adenylyl cyclase. Species Crossreactivity: human, bovine, porcine, rat, mouse, guinea pig, gerbil and frog.
