Thymidylate Synthase (TS)1 (EC 2.1.1.45) converts deoxyuridine monophosphate (dUMP) to deoxythymidine monophosphate (dTMP), which is essential for DNA biosynthesis.
TS is a critical target for the fluoropyrimidines, an important group of antineoplastic drugs that are widely used in the treatment of solid tumors.
Both 5-FU and fluorodeoxyuridine are converted in tumor cells to FdUMP which inactivates TS by formation of a ternary covalent complex in the presence of the folate cofactor 5,10-methylenetetrahydrofolate.
Expression of TS protein is associated with response to 5-fluorouracil (5-FU) in human colorectal,4,5 gastric,5 head and neck,6 and breast7 carcinomas.
Applications:Suitable for use in ELISA, Flow Cytometry, Immunofluorescence, Immunoprecipitation and Western Blot.
Not suitable for use in Immunohistochemistry.
Other applications have not been tested.
Recommended Dilution:Immunoprecipitation (Native verified) (Use Protein G): 2ug/mg protein lysate
Western Blot: 1-2ug/ml for 2hrs at RT
Optimal dilutions to be determined by the researcher.
Positive Control:5-FU-resistant cell cancer lines or colon carcinoma.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：500ul
Host：mouse
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein G affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.2% BSA, 0.1% sodium azide. Also available without BSA and azide. See T5155-10X.
Specificity：Recognizes human Thymidylate Synthase
Clone # USB：TS106 (0.N.569)
Isotype：IgG1,k
Calc Applications Abbrev：E FC IF IP WB
Calc Crossreactivity：Hu
Immunogen：Recombinant human TS enzyme (36kD). Cellular Localization: Nuclear and cytoplasmic
