85-7208-50　［取扱停止］Thymidine Phosphorylase (TYMP, TP, ECGF1, Gliostatin, hPD-ECGF, MNGIE, PDECGF, PD-ECGF, Platelet-derived endothelial cell growth factor, TdRPase) 500ul　T5152-10

特徴

• In the presence of inorganic orthophosphate, thymidine phosphorylase catalyses the reversible phospholytic cleavage of thymidine and deoxyuridine to their corresponding bases and 2-deoxyribose-1-phosphate.
• It is both chemotactic and mitogenic for endothelial cells and a non-heparin binding angiogenic factor present in platelets.
• Its enzymatic activity is crucial for angiogenic activity (metabolite is angiogenic).
• Higher levels of serum TP/PD-ECGF are observed in cancer patients.
• It is also involved in transformation of fluoropyrimidines, cytotoxic agents used in the treatment of a variety of malignancies, into active cytotoxic metabolites (e.g.
• 5'-deoxy-5-fluorouridine to 5-FU).
• High intra-cellular levels of TP/PD-ECGF are associated with increased chemosensitivity to such antimetabolites.
• Applications:Suitable for use in Immunohistochemistry.
• Other applications have not been tested.
• Note: As reported by an independent laboratory, neutralizes enzymatic activity of TP/PD-ECGF.
• Recommended Dilution:Immunohistochemistry (FFPE): 1-2ug/ml for 30 minutes at RT.
• No special treatment is required for immunohistochemical staining of formalin fixed tissues.
• Optimal dilutions to be determined by the researcher.
• Positive Control:HUVEC cells or breast carcinoma
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.