The Thomsen-Fridenreich antigen acts as an oncofetal antigen, with low expression in normal adult tissues but increasing to fetal levels of expression in hyperplasia or malignancy.2-4 It is considered as a pan-carcinoma marker.
Can be applied for the detection of cells with TF-antigen and is especially applicable for sensitive determination of neuraminidases.
Applications:Suitable for use in Immunofluorescence and Immunohistochemistry.
Other applications have not been tested.
Recommended Dilution:Immunohistochemistry (Formalin/paraffin): 1-2ug/ml for 30 min at RT.
Optimal dilutions to be determined by the researcher.
Positive Control:KG1 cells and human colorectal carcinoma tissues.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：500ul
Host：mouse
Source Antibody：human
Grade：Purified
Purity：Purified by ammonium sulfate precipitation.
Form：Supplied as a liquid in 10mM PBS, pH 7.4, 0.2% BSA, 0.09% sodium azide.
Specificity：Recognizes human and rat Thomsen-Friedenreich Antigen is specific for both anomeric forms of the disaccharide (TFa and TFb, including related structures on the glycolipid) and shows no crossreactivity with sialylated glycophorin.
Clone # USB：0.N.567 (A78-G/A7)
Isotype：IgM,k
Calc Applications Abbrev：IF IHC
Calc Crossreactivity：Hu Rt
Immunogen：Neuraminidase-treated human red blood cells. Epitope: Galb1-3GalNAc. Cellular Localization: Cell membrane.
