TdT is a template-independent DNA polymerase located in the cell nucleus which catalyses irreversible terminal addition, via polymerization, of deoxynucleotides at the 3' hydroxyl ends of oligo or polydeoxynucleotide initiators.
TdT is considered to be a highly specific marker for the diagnosis and classification of acute lymphoblastic lymphoma/leukemias.
The determination of TdT expression is most valuable when it is different to differentiate histologically between lymphoblastic lymphoma and Burkitt’s lymphoma.
Applications:Suitable for use in Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Immunohistochemistry (Formalin/paraffin): 1:20-1:30 for 60 min at RT.
Staining of formalin-fixed tissues REQUIRES boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.
Optimal dilutions to be determined by the researcher.
Positive Control:Thymus
Cellular Localization:Nuclear
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：500ul
Host：mouse
Source Antibody：human
Grade：Supernatant
Purity：Tissue culture supernatant.
Form：Supplied as liquid in 0.09% sodium azide.
Specificity：Recognizes human TdT. Does not crossreact with rat.
Clone # USB：0.N.564
Isotype：IgG2a
Calc Applications Abbrev：IHC
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to the amino terminal region of the terminal deoxynucleotidyl transferase molecule.
