S100 belongs to the family of calcium binding proteins such as calmodulin and troponin C.
S100A is composed of an alpha and beta chain whereas S100B is composed of two beta chains.
Antibody to S100 stains Schwannomas, ependymomas, astrogliomas, almost all benign and malignant melanomas and their metastases.
S100 protein is also expressed in the antigen presenting cells such as the Langerhans cells in skin and interdigitating reticulum cells in the paracortex of lymph nodes.
S100 is a nervous tissue specific soluble protein made in glial cells.
Present in sweat glands chongrocytes and myoepithelial cells.
May be found as cytoplasmic, membranous, or mixed throughout the areas of tumor.
Usually cytoplasmic when present in normal tissues.
The diagnosis of Histocytosis X is confirmed by S100 staining.
S100 protein is highly soluble and may be eluted from frozen tissue duing staining.
Applications:Suitable for use in Immunohistochemistry.
Other applications not tested.
Recommended Dilutions:Immunohistochemistry (Formalin/paraffin): 1:100 for 20 minutes at RT.
No special pretreatment is required for formalin/paraffin tissues.
Optimal dilution determined by the researcher.
Positive Control:Melanoma or Schwannoma
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：500ul
Host：mouse
Source Antibody：bovine
Grade：Affinity Purified
Purity：Purified by Protein A affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.2% BSA, 0.09% sodium azide. Also available without BSA and azide. See S0052-06X.
Specificity：Recognizes bovine S100. Species Crossreactivity: human, rat and mouse.
