Rb is a tumor suppressor nuclear phosphoprotein capable of binding to DNA.
It is phosphorylated on serine and threonine, but not tyrosine residues.
It forms a complex with SV40 large T antigen, adenovirus E1A, and human papilloma virus-16E.
Rb protein may act by regulating transcription and loss of its function leads to uncontrolled cell growth.
Aberrations in the Rb gene have been implicated in cancers of breast, colon, prostate, kidney, nasopharynx, and leukemia.
Applications:Suitable for use in Flow Cytometry, Immunofluorescence, Immunoprecipitation, Western Blot and Immunohistochemistry.
Other applications have not been tested.
Recommended Dilution:Immunoprecipitation (native and denatured): 2ug/mg protein lysate.
Use Protein G.
Western Blot: 1-2ug/ml for 2hrs at RT.
Immunohistochemistry (formalin/paraffin): 1-2ug/ml for 30 min at RT.
Staining of formalin-fixed tissues REQUIRES boiling tissue sections in 1mM EDTA, pH 8.0, for 10-20 min followed by cooling at RT for 20 min.
EDTA is better than citrate for epitope unmasking.
Optimal dilutions to be determined by the researcher.
Positive Control:LS174T, HT29, HUVEC cells.
Normal colon and carcinoma.
Breast carcinoma.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：500ul
Host：mouse
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein G affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.2% BSA, 0.09% sodium azide. Also available without BSA and azide. See R1660-18X.
Specificity：Recognizes human Retinoblastoma (Rb). Reacts with hyperphosphorylated and un (under) phosphorylated form of Rb. Specifically stains the nuclei of BT-20 cells and primary human foreskin fibroblast (HFF) cells. Does not react with p107 or p130. Does not crossreact with mouse or rat.
Clone # USB：0.N.544 (1F8)
Isotype：IgG1
Calc Applications Abbrev：FC IF IHC IP WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to aa703-722 from human Retinoblastoma protein. Cellular Localization: Nuclear.
