85-7208-44　Renal Cell Carcinoma 500ul　R1450

特徴

• In normal kidney, gp200 is localized along the brush border of the pars convoluta and pars recta segments of the proximal tubule, as well as focally along the luminal surface of Bowman’s capsule adjoining the outgoing proximal tubule.
• Of other normal tissues examined, the gp200 is also localized along the luminal surfaces of breast lobules and ducts, the luminal surface of the epididymal tubular epithelium, within the cytoplasm of parathyroid parenchymal cells, and focally within the colloid of thyroid follicles.
• Thirty-one other normal tissues failed to express similar or crossreacting antigens.
• Reportedly, gp200 is expressed by 93% of primary and 84% of metastatic renal cell carcinomas.
• Useful reagent in the investigations of carcinomas of proximal nephrogenic differentiation especially those showing tubular differentiation.
• Applications:Suitable for use in Western Blot and Immunohistochemistry.
• Other applications not tested.
• Recommended Dilutions:Immunohistochemistry (Formalin/paraffin): 2-4ug/ml for 30 minutes at RT.
• Staining of fromalin-fixed tissues requires digestion with Protesse XXV at 1mg/ml PBS for 10 minutes at RT.
• Western Blot: Reduced & non-reduced
• Optimal dilutions to be determined by the researcher.
• Positive Control:Normal kidney or renal cell carcinoma
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.