Prostatic acid phosphatase (PSAP) is one of the two antigenic markers of prostatic carcinoma, the other being prostate specific antigen.
It belongs to the kallikrein family of serine proteases and is suggested to act as a hydrolase to split phospharyl choline in semen and as a transferase.
Applications:Suitable for use in Immunofluorescence and Immunohistochemistry.
Other applications have not been tested.
Recommended Dilution:Immunohistochemistry (Frozen & formalin/paraffin): 0.25-0.5ug/ml for 30 min at RT.
No special pretreatment is required for immunohistochemical staining of formalin/paraffin tissues.
Optimal dilutions to be determined by the researcher.
Positive Control:Normal prostate or prostate carcinoma
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：500ul
Host：mouse
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein G affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.2% BSA, 0.09% sodium azide.
Specificity：Recognizes a protein of 52kD, identified as prostate specific acid phosphatase (PSAP). Highly specific to PSAP and shows no crossreaction with other phosphatases. Reacts with non-neoplastic adult and fetal prostatic glands, primary and metastatic prostatic carcinomas. Does not inhibit the enzymatic activity of PSAP. Species Crossreactivity: Human. Does not react with canine, rabbit and rat.
Clone # USB：0.N.539
Isotype：IgG1
Calc Applications Abbrev：IF IHC
Calc Crossreactivity：Hu
Immunogen：Purified prostatic acid phosphatase (PSAP) from human seminal plasma. Cellular Localization: Cytoplasmic.
