Human PgR exists in two forms: 116kD (B-form) and 81kD (A-form).
It acts as ligand activated transcription factor to regulate expression of the target genes.
Null mutation in PgR gene leads to pleiotrophic reproductive abnormalities.
Applications:Suitable for use in Immunofluorescence, Western Blot, Immunoprecipitation and Sedimentation in sucrose gradients.
Other applications not tested.
Recommended Dilutions:Immunoprecipitation (Native): 2ug/mg protein lysate using Protein G.
Western Blot: 1-2ug/ml for 2 hours at RT.
Optimal dilutions to be determined by the researcher.
Positive Control:T47D cells
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：500ul
Host：mouse
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein G affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.2% BSA, 0.09% sodium azide.
Specificity：Equally recognizes two proteins: 116kD (triplet) and 81kD (singlet) which are identified as the hormone-binding high (B) and low (A) MW forms of human progesterone receptor. Species Crossreactivity: baboon, monkey, mouse and rat. Does not crossreact with guinea pig.
Clone # USB：0.N.528 (hPRa7)
Isotype：IgG1,k
Calc Applications Abbrev：IF IP WB
Calc Crossreactivity：Hu Mk Mo Rt
Immunogen：Progesterone Receptor from a human endometrial carcinoma (EnCa 101) grown in athymic mice. Epitope: N-terminal half of human PgR. Cellular Localization: Nuclear
