Applications:Suitable for use in ELISA and Immunohistochemistry.
For coating ELISA use P4214-10X.
Other applications have not been tested.
Recommended Dilution:Immunohistochemistry (Formalin/paraffin): 1:200 for 20 minutes at RT.
Staining of formalin-fixed tissues requires digestion with Protease XXV at 1mg/ml PBS for 5 minutes at 37°C.
Optimal dilutions to be determined by the researcher.
Cellular Localization:Cell membrane.
Positive Control:Placenta
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size:500ul
Host:mouse
Source Antibody:human
Grade:Affinity Purified
Purity:Purified by Protein A affinity chromatography from ascites.
Form:Supplied as a liquid in 10mM PBS, pH 7.4, 0.2% BSA, 0.09% sodium azide. Also available without BSA and Azide. See P4214-10X.
Specificity:Recognizes a membrane-bound isoenzyme (Regan and Nagao type) of Placental Alkaline Phosphatase (PLAP) occuring in the placenta during the 3rd trimester of gestation.