Elevated expression of PCNA/cyclin has been shown in the nucleus during late G1 phase immediately before the onset of DNA synthesis, becoming maximal during S-phase and declining during G2 and M phases.
Proliferating cell nuclear antigen (PCNA) is a 36kD nuclear protein associated with the cell cycle, a nuclear protein vital for cellular DNA synthesis.
Proliferating cell nuclear antigen was originally identified by immunofluorescence as a nuclear protein whose appearance correlated with the proliferate state of the cell.
PCNA is required for replication of DNA in vitro and has been identified as the auxiliary protein (cofactor) for DNA polymerase delta.
The anti-PCNA antibodies react with the nuclei of proliferating cells.
PCNA is essential for cellular DNA synthesis and is also required for the in vitro replication of simian virus 40 (SV40) DNA where it acts to coordinate leading and lagging strand synthesis at the replication fork.
The PCNA protein may fulfil several separate roles in the cell nucleus associated with changes in its antigenic structure.
Applications:Suitable for use in Flow Cytometry, Immunoprecipitation, Western Blot and Immunohistochemistry.
Other applications have not been tested.
Recommended Dilution:Immunoprecipitation (Native and denatured): 2ug/mg protein lysate.
Use Protein A.
Western Blot: 1:50 for 2hrs at RT.
Immunohistochemistry (Formalin/paraffin): 1:400-1:800 for 20 minutes at RT.
Formalin-fixed tissues require boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10-20 minutes followed by cooling at RT for 20 minutes.
Optimal dilutions to be determined by the researcher.
Positive Control:
Raji cells.
Tonsil, lymph node, or small intestine
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：500ul
Host：mouse
Source Antibody：rat
Grade：Affinity Purified
Purity：Purified by Protein A affinity chromatography from ascites.
Form：Supplied as a liquid in 10mM PBS, pH 7.4, 0.2% BSA, 0.09% sodium azide.
Specificity：Recognizes rat proliferating cell nuclear antigen (PCNA). Species Crossreactivity: human, mouse, insect and yeast.
Clone # USB：0.N.508 (PC10)
Isotype：IgG2a,k
Calc Applications Abbrev：FC IHC IP WB
Calc Crossreactivity：Hu Mo Rt Ye
Immunogen：Recombinant protein corresponding to rat PCNA.
