85-7208-34　MDR1 (Multi Drug Resistance Protein 1, ABC20, ATP Binding Cassette Subfamily B (MDR/TAP) Member 1, ABCB1, CD243, CLCS, GP170, P-Glycoprotein, P-gp, P Glycoprotein 1, PGY1) 500ul　M2825-14B

特徴

• Shows no crossreactivity with the human MDR3 product.
• Its epitope localizes in the extracellular amino terminal half of P-glycoprotein (at the third extracellular loop of the molecule).
• This epitope is resistant to formalin fixation and periodate oxidation1 and is different from those of other commonly known Mabs to P-glycoprotein, such as C219, JSB-1, and MRK16, which recognize an intracellular and extracellular epitope of P-glycoprotein, respectively.
• Detects soluble P-glycoprotein in the cultured media of viable adriamycin-resistant cells, whereas those of the drug-sensitive parent cells contain no detectable level of soluble P-glycoprotein.
• Soluble P-glycoprotein is also detected in extracellular fluids of cancer patients, such as malignant ascites and serum.
• The Mr of soluble P-glycoprotein is the same as that of membrane bound P-glycoprotein.
• Applications:Suitable for use in Flow Cytometry, Immunofluorescence, Immunohistochemistry and Western Blot.
• Other applications have not been tested.
• Recommended Dilutions:Flow Cytometry: Fixed cells only.
• Western Blot: 1-2ug/ml for 2hrs at RT
• Immunohistochemistry: 2-4ug, for 30 minutes at RT.
• Staining of formalin-fixed tissues REQUIRES boiling tissue sections in 1mM EDTA, pH 8.0 for 10-20 minutes followed by cooling at RT for 20 minutes.
• Optimal dilutions to be determined by the researcher.
• Positive Control:Drug resistant cells, Kidney or breast carcinoma.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.